PfGRP170 Interacts with BiP (c) In vivo interaction of PfGRP170 and BiP. PfGRP170‐GFP‐DDD parasites were paraformaldehyde fixed and stained with anti‐GFP and anti‐BiP. A proximity ligation assay (PLA) was then performed. The scale bar is 5 μm. A negative control using anti‐GFP and anti‐PfPMV is shown in (d). (e) Asynchronous PfGRP170‐GFP‐DDD parasites overexpressing PfBiP‐Ty1 were paraformaldehyde fixed and stained with anti‐GFP (PfGRP170), anti‐Ty1 (PfBiP‐Ty1‐KDEL), and DAPI to visualise the nucleus. The images were taken with Delta Vision II, deconvolved, and are displayed as a maximum intensity projection. The scale bar is 5 μm. Each data point is representative of the mean of three replicates ± SEM,

Kudyba HM, Cobb DW, Fierro MA, Florentin A, Ljolje D, Singh B, Lucchi NW, Muralidharan V. The endoplasmic reticulum chaperone PfGRP170 is essential for asexual development and is linked to stress response in malaria parasites. Cell Microbiol. 2019 21(9):e13042.