Plasmodium falciparum RASP2 expression and localisation in erythrocytic stage. a IFAs on PfRASP2-HA3 schizonts showing PfRASP2-HA3 with rhoptry markers. RON4, rhoptry neck, RhopH2, rhoptry bulb. b Comparison of the expression patterns of known rhoptry proteins during the P. falciparum erythrocytic cycle in combination with the expression profile of PF3D7_0210600 (PfRASP2). Real-time transcription data of the P. falciparum 3D7 strain obtained by biosynthetic pyrimidine labeling (PlasmoDB.org). c IFAs of PfRASP2 in free merozoites. d IFAs of invading merozoites. Left, extracellular parasite attached to red blood cell. Middle, at early point of invasion, the RON4 marker detects the moving junction at the posterior end of the intracellular merozoite, while RASP2 remains apical. Right, export of the bulb marker RhopH2 upon invasion. c Pictures of individual merozoites where acquired with a confocal microscope with Airyscan. Suarez C, Lentini G, Ramaswamy R, Maynadier M, Aquilini E, Berry-Sterkers L, Cipriano M, Chen AL, Bradley P, Striepen B, Boulanger MJ, Lebrun M. A lipid-binding protein mediates rhoptry discharge and invasion in Plasmodium falciparum and Toxoplasma gondii parasites. Nat Commun. 2019 10(1):4041.