DPAP3 is expressed during schizogony and localizes at the apical pole. (D) IFAs of DPAP3-mCh, DPAP3-HA, and DPAP3-GFP mature schizonts stained with anti-MSP1 (red), and anti-mCherry, -HA, or -GFP (green) antibodies (confocal microscopy). (F) IFA of DPAP3-HA schizonts collected 39–48 h.p.i. and stained with anti-HA (green) and anti-AMA1 (microneme), anti-SUB1 (exoneme), anti-RON4 (rhoptry neck), or anti-RopH2 (rhoptry bulb) antibodies (all red). (H) IFA of C2-arrested DPAP3-HA schizonts analyzed by SIM. Parasites were stained with anti-HA (green) and anti-SUB1, anti-EBA175, or anti-RON4 antibodies (all red). Representative 3D-reconstruction images of merozoites are shown. In all IFAs DNA was stained with DAPI (blue); scale bars = 5 μm except for merozoite images in H (1 μm). Lehmann C, Tan MSY, de Vries LE, Russo I, Sanchez MI, Goldberg DE, Deu E. Plasmodium falciparum dipeptidyl aminopeptidase 3 activity is important for efficient erythrocyte invasion by the malaria parasite. PLoS Pathog. 2018 PMID: 29768491.