Schizont and merozoite culture was incubated overnight with 0.1µM cytochalasin D to inhibit invasion. Thin smeared on a glass slide, fixed in methanol, stained with rabbit anti-Profilin polyclonal serum 1:300 and secondary Alexa-Flor488 anti-Rabbit. Dual color fluorescence images were captured using a Carl Zeiss Axioskop 2 microscope at 100x using Oil immersion. Nucleus is marked with DAPI. Scale bar indicate 1µM. Profilin is cytoplasmic but has a trend of localization towards the apex of merozoite.
Baum J, Tonkin CJ, Paul AS, Rug M, Smith BJ, Gould SB, Richard D, Pollard TD, Cowman AF. A malaria parasite formin regulates actin polymerization and localizes to the parasite-erythrocyte moving junction during invasion. Cell Host Microbe. 2008 Mar 13;3(3):188-98.