Native PfRA expression was analyzed by confocal immunofluorescence microscopy. Immunofluorescence assays were performed on fixed smears of different stages of the P. falciparum intra-erythrocytic life cycle. PfRA (green) staining was detected by the anti-PfRA mouse antibodies only in the schizont stages. No signal was observed in trophozoites and schizonts. A distinct punctate staining of PfRA was observed in the schizont which is characteristic of apically localized
Anand G, Reddy KS, Pandey AK, Mian SY, Singh H, Mittal SA, Amlabu E, Bassat Q, Mayor A, Chauhan VS, Gaur D. A novel Plasmodium falciparum rhoptry associated adhesin mediates erythrocyte invasion through the sialic-acid dependent pathway. Sci Rep. 2016 6:29185. PMID: 27383149;