PfCen1-4 localize to centriolar plaque and can display liquid-like dynamics. (A) Schematic of nuclear and cell division during asexual blood stage schizogony (B) Schematic of PfCen1-4 and HsCen2 indicating reported post-translational modifications and EFh domains. (C) Immunofluorescence staining of tubulin and PfCen1-4-GFP in parasite strains. All images are maximum intensity projections (MIP). DNA stained with Hoechst. Scale bars; confocal, 1 μm, STED, 100 nm.(D-E) STEDtimelapse of centriolar plaque region of parasites expressing PfCen1-Halo labeled with MaP-SiR-Halo dye. DNA stained with SPY505-DNA. Scale bar, 100 nm. Quantification of ratio (black line) between height and width (grey lines) of the PfCen1-Halo signal, as indicated in the small schematic. Voß Y, Klaus S, Lichti NP, Ganter M, Guizetti J. Malaria parasite centrins can assemble by Ca2+-inducible condensation. PLoS Pathog. 2023 19(12):e1011899. PMID: 38150475
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Other associated proteins
PFID | Formal Annotation |
---|---|
PF3D7_0107000 | centrin-1 |
PF3D7_1027700 | centrin-3 |
PF3D7_1446600 | centrin-2 |