The FP2a 120 aa reporter shows significantly more association with EXP2 upon WR99210 trapping.

(A) Highly synchronous parasites expressing the 120 aa, 190 aa and NT FP2a reporters were sorbitol synchronised at ring stage and treated ± 10 nM WR99210 to prevent unfolding of the reporters to see if they would become trapped at the parasite periphery with PTEX. When treated with WR99210, more 120 aa reporter was observed at the PPM/PVM indicating its trafficking was affected. No substantial difference was observed for 190 aa or NT reporters. Shown are representative images for 3 (120 aa) and 2 (190 aa and NT) biological replicates. Rabbit anti-EXP2 was used as a PVM marker, rabbit anti-Nluc to detect the reporters and DAPI to stain the nucleus. FV = food vacuole. Scale bars = 5 μm. Arrows point to PVM loops. Jonsdottir TK, Elsworth B, Cobbold S, Gabriela M, Ploeger E, Parkyn Schneider M, Charnaud SC, Dans MG, McConville M, Bullen HE, Crabb BS, Gilson PR. PTEX helps efficiently traffic haemoglobinases to the food vacuole in Plasmodium falciparum. PLoS Pathog. 2023 19(7):e1011006.