PF3D7_1141600 dolichol-phosphate mannosyltransferase

Detection of proteins in blood stage parasites was performed using indirect fluorescent-antibody tests. Thin films of parasites at mature stages were fixed in cold acetone and incubated sequentially with 1/80 dilutions of sera from peptide-immunized mice plus rabbit anti-ERC1 (1:200) to determine localization to the parasite ER. These slides were washed and incubated with an appropriate mixture of fluorescein-conjugated anti-mouse and rhodamine-conjugated anti-rabbit (1:1.000) antibodies. Slides were photographed under appropriate illumination for fluorescein isothiocyanate (first column, showing PIG-A, PIG-O, or DPM1 localization) and rhodamine (second column, showing ERC1 localization), and photographs were overlaid (third column).

Delorenzi M, Sexton A, Shams-Eldin H, Schwarz RT, Speed T, Schofield L. Genes for glycosylphosphatidylinositol toxin biosynthesis in Plasmodium falciparum. Infect Immun. 2002 70:4510-22. PMID:

Other associated proteins

PFID Formal Annotation
PF3D7_1032400 phosphatidylinositol N-acetylglucosaminyltransferase subunit A, putative
PF3D7_1108600 endoplasmic reticulum-resident calcium binding protein
PF3D7_1214100 GPI ethanolamine phosphate transferase 3, putative
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