VAR2CSA expression and localisation of transgenic NF54CSA T934A and T934D parasites. (Left panels) VAR2CSA cell surface staining of live parasites examined by immunofluorescence using a rabbit anti-VAR2CSA immunopurified antibody followed by a Goat Alexa Fluor 488 anti-rabbit. The nucleus was stained using Hoesch dye. (Right panels) VAR2CSA staining on fixed and permeabilised smears after labelling with a rat serum anti-VAR2CSA followed by a goat Alexa Fluor anti-rat. The nucleus was observed with DAPI. Scale bar represents 5 μM. BF, bright field; iRBC, infected red blood cell.
Dorin-Semblat D, Tétard M, Claës A, Semblat JP, Dechavanne S, Fourati Z, Hamelin R, Armand F, Matesic G, Nunes-Silva S, Srivastava A, Gangnard S, Lopez-Rubio JJ, Moniatte M, Doerig C, Scherf A, Gamain B. Phosphorylation of the VAR2CSA extracellular region is associated with enhanced adhesive properties to the placental receptor CSA. PLoS Biol. 2019 17(6):e3000308.