Lsm7 effects on SG formation. Scale bar indicates 2 μm. If not stated otherwise, stress was induced by 2 h 2-DG treatment and >200 cells were analyzed for each biologically independent experiment. Values represent percentage of foci formation or co-localization and are shown as mean ± S.D. a Deletion of LSM7 decreases SG formation as compared to the control (his3Δ). Seven biologically independent experiments were examined (unpaired twotailed t-test). ****p < 0.0001. b Overexpression of LSM7 increases SG formation in the WT (BY4741) strain as compared to empty vector control. No SG induction under unstressed conditions. Six biologically independent experiments were examined (unpaired two-tailed t-test). ****p < 0.0001, ns =0.3317. c The Pab1 protein expression levels were not changed in the lsm7Δ or the LSM7OE strain. Data are representative of three biologically independent experiments. d Deletion of LSM7 affects SG formation under glucose starvation and stationary phase, as compared to the control (his3Δ). Six biologically independent experiments were examined and >300 cells were analyzed for each (unpaired two-tailed t-test). Left to right: ***p = 0.0003, ****p < 0.0001, ns = 0.0519, ns = 0.4126. e Lsm7 foci co-localize with SGs to a greater extent than other Lsm1-7/Pat1 complex components. Three biologically independent experiments were examined (one-way ANOVA followed by Dunnett’s test). Left to right: ****p < 0.0001, ***p= 0.0007, ***p = 0.0003, **p = 0.0018, ***p =0.001, **p = 0.0036. f Deletion of some Lsm1-7/Pat1 components results in decreased SG formation. Seven (his3Δ, lsm1Δ, pat1Δ), six (lsm6Δ), five (lsm4ΔC), and three (lsm2Δ(TS)) biologically independent experiments were examined (one-way ANOVA followed by Dunnett’s T3 multiple comparisons test, with individual variances computed for each comparison). Left to right: ****p < 0.0001, ns = 0.3105, ns = 0.5875, ns=0.9983, ***p = 0.0006. g Deletion of both LSM7 and LSM1 results in greater loss of SGs than the lsm1Δ single deletion. Seven (lsm1Δ) and three (lsm1Δlsm7Δ) biologically independent experiments were examined (unpaired two-tailed t test with Welch’s correction). **p = 0.003. h Overexpression of LSM8 affects Lsm7 foci and SG formation as compared to empty vector control. Four biologically independent experiments were examined (unpaired two-tailed t test). Left to right: **p = 0.007, ***p = 0.0002, ns = 0.096. Lindström M, Chen L, Jiang S, Zhang D, Gao Y, Zheng J, Hao X, Yang X, Kabbinale A, Thoma J, Metzger LC, Zhang DY, Zhu X, Liu H, Gustafsson CM, Burmann BM, Winderickx J, Sunnerhagen P, Liu B. Lsm7 phase-separated condensates trigger stress granule formation. Nat Commun. 2022 13(1):3701. PMID: 35764627