PfmtRPS18 is likely localized to the mitochondrial inner membrane. (A), (B) Images of D10-PfmtRPS18-3HA parasites. RBC, red blood cell. PVM, parasitophorous vacuole membrane. PPM, parasite plasma membrane. DV, digestive vacuole. ER, endoplasmic reticulum. M, mitochondrion. Black dots in red boxes indicate PfmtRPS18-3HA is localized to the mitochondrial inner membrane. (C) A control with omission of the primary antibody shows clear backgound. Mag, magnification. Data shown are representative of two biological experiments. PfmtRPS18 was likely associated with the MIM. As a negative control, omission of the primary antibody had a clean background (C). Together, these data suggest the Plasmodium mitoribosome is likely bound to the mitochondrial inner membrane, as in other organisms.

Ling L, Mulaka M, Munro J, Dass S, Mather MW, Riscoe MK, Llinás M, Zhou J, Ke H. Genetic ablation of the mitoribosome in the malaria parasite Plasmodium falciparum sensitizes it to antimalarials that target mitochondrial functions. J Biol Chem. 2020 Apr 9. pii: jbc.RA120.012646