Subcellular localization of known and putative iron transport proteins. Representative erythrocytes infected with P. falciparum 3D7 parasites endogenously expressing GFP-tagged PfMRS3 (A), PfVIT (B), PfZIPCO (C) or PfE140 (D) were additionally stained with the fluorescent dyes Hoechst-33342, MitoTracker Red, ER Tracker Red and/or LysoTracker Deep Red. Co-transfection with a construct that encodes the 60 N-terminal amino acids of acyl carrier protein (PfACP) tagged with mCherry (Birnbaum et al., 2020) resulted in red fluorescence of the apicoplast. Live-cell images were taken under physiological conditions at 37°C using an SP8 confocal laser-scanning microscope (Leica). DIC, differential interference contrast. Scale bar, 2 µm. Wunderlich J, Kotov V, Votborg-Novél L, Ntalla C, Geffken M, Peine S, Portugal S, Strauss J. Iron transport pathways in the human malaria parasite Plasmodium falciparum revealed by RNA-sequencing. Front Cell Infect Microbiol. 2024 Nov 7;14:1480076. doi: 10.3389/fcimb.2024.1480076. PMID: 39575308
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Other associated proteins
PFID | Formal Annotation |
---|---|
PF3D7_0104100 | protein e140, putative |
PF3D7_0208500 | acyl carrier protein |
PF3D7_1022300 | ZIP domain-containing protein, putative, ZIPCO |