Single-staining IFA of 3D7, T996, and FCB1 parasites. Primary antibodies were polyclonal mouse antisera RH1.1, RH1.2, RH2a.1, and RH2b.1. Secondary antibodies were FITC-conjugated anti-mouse immunoglobulin G (Sigma). The parasite nuclei are stained with DAPI. Scale bar, 10 μm. Taylor HM, Grainger M, Holder AA. Variation in the expression of a Plasmodium falciparum protein family implicated in erythrocyte invasion. Infect Immun. 2002 PMID: 12228308