Gene transcription and spatial distribution of the PF3D7_1372300 protein. Indirect immunofluorescence of PF3D7_1372300. Rabbit anti-PF3D7_1372300 antibody served as the primary antibody, IgG from healthy rabbits was used as a negative control, and buffer without antibody served as a blank control. Parasite nuclei were stained with DAPI. The IFA results revealed dotted fluorescence on the membrane of iRBCs but also in the cytoplasm, during the early stage of

infection. As parasites developed, expression of the protein became more extensive. The fluorescence was distributed throughout the iRBCs, and the fluorescence which was located close to the iRBCs membrane was stronger than that in the cytoplasm of the iRBCs.

Yang B, Wang X, Jiang N, Sang X, Feng Y, Chen R, Wang X, Chen Q. Interaction Analysis of a Plasmodium falciparum PHISTa-like Protein and PfEMP1

Proteins. Front Microbiol. 2020 Nov 13;11:611190.