PfAP2-G2 is expressed from around 16-19 hpi in asexual stage parasite. Live fluorescence microscopy performed on a highly synchronized population of AP2-G2::GFP parasites every 7 h of the asexual life cycle showed that PfAP2-G2::GFP colocalizes with the nucleus in both the trophozoite and schizont stages. DRAQ-5 was used as the nuclear stain.
Singh S, Santos JM, Orchard LM, Yamada N, van Biljon R, Painter HJ, Mahony S, LlinĂ¡s M. The PfAP2-G2 transcription factor is a critical regulator of gametocyte maturation. Mol Microbiol. 2020 Dec 25.