IFA analysis of PfAP2-G2 during asexual parasite development. PfAP2-G2 localization was identified by using the anti-Flag antibody (red), while nuclei were counterstained with DAPI. Scale bars, 5 μm. IFA of fixed asexual parasites detected that at the ring stage PfAP2-G2 was shown as a single punctum at the nuclear periphery demarcated by DAPI staining, while in the trophozoite stage, it diffused and Overlapped more extensively with the nucleus. However, the anti-Flag staining became much fainter at the schizont stage and the more prominent foci did not overlap with the DAPI staining.
Xu Y, Qiao D, Wen Y, Bi Y, Chen Y, Huang Z, Cui L, Guo J, Cao Y. PfAP2-G2 Is Associated to Production and Maturation of Gametocytes in Plasmodium falciparum via Regulating the Expression of PfMDV-1. Front Microbiol. 2021 11:631444.