PfMCMBP-deficient parasites complete budding and form merozoites with varied cell body size and ploidy. (A) Representative pictures from [+]/[−] Shld1 PfMCMBP3HADD parasites treated with E64, stained with SYBR Green I and probed with rabbit anti-PfGAP45. Images were taken with E64-treated schizonts were stained with anti-PfGAP45 (an inner membrane complex-associated protein, and SYBR Green I as a marker of the merozoite membrane and nuclear DNA, respectively. Strikingly, at this late fully-segmented stage, the nuclear morphology in the PfMCMBP-deficient parasites resembled the [+] Shld1 condition, and we no longer observed scattered DNA.
Absalon S, Dvorin JD. Depletion of the Mini-Chromosome Maintenance Complex Binding Protein allows the progression of cytokinesis despite abnormal karyokinesis during the asexual development of Plasmodium falciparum. Cell Microbiol. 2020 Oct 30:e13284.
Other associated proteins
PFID | Formal Annotation |
---|---|
PF3D7_1222700 | glideosome-associated protein 45 |