PfGEST localization in the P. falciparum gametocyte osmiophilic bodies. B, IFA on acetone-fixed wt gametocytes using mouse a-PfGEST (1:100 dilution) and rabbit a-Pfg377B (1:800 dilution) antibodies. Scale bar: 5 mM. BF: bright field. a-PfGEST positive signal colocalized with the specific granular fluorescent pattern obtained with antibodies against the OB marker Pfg377, strongly indicating that PfGEST resides in these organelles C, Representative immunoelectron micrograph showing the localization of PfGEST specific gold particles (a-PfGEST primary antibody 1:100 dilution) on the electrondense OB structures in sections from wt gametocytes. Scale bar: 1 mM.
Suárez-Cortés P, Sharma V, Bertuccini L, Costa G, Bannerman NL, Sannella AR, Williamson K, Klemba M, Levashina EA, Lasonder E, Alano P. Comparative Proteomics and Functional Analysis Reveal a Role of Plasmodium falciparum Osmiophilic Bodies in Malaria Parasite Transmission. Mol Cell Proteomics. 2016 15(10):3243-3255.
Other associated proteins
PFID | Formal Annotation |
---|---|
PF3D7_1250100 | osmiophilic body protein g377 |