Expression and OB localization of the PfSUB2-, PfDPAP2- and PfOBC13-GFP fusions in late gametocytes. A, GFP fluorescence
of live gametocytes from the lines indicated. B, IFA experiments with a-GFP (rabbit, 1: 100, green) used alone or in combination with rat a-Pfg377-A2 (1:200 dilution) antibodies on gametocytes from the lines indicated. BF: bright field. Scale bar: 5 mM. The a-GFP antibody produced a granular fluorescence pattern in gametocytes of the pfsub2GFP, pfdpap2GFP and pfobc13GFP lines (left). A different pattern was found instead in the pfpsop12GFP line, with gametocytes showing in some experiments a more condensed cytoplasmic signal, or no signal at all (data not shown). Importantly,
the GFP signals in the gametocytes of 3D7pfsub2GFP,
3D7pfdpap2GFP, and 3D7pfobc13GFP transgenic clones colocalized
with those of the OB marker Pfg377 and of PfGEST (right),
Suárez-Cortés P, Sharma V, Bertuccini L, Costa G, Bannerman NL, Sannella AR, Williamson K, Klemba M, Levashina EA, Lasonder E, Alano P. Comparative Proteomics and Functional Analysis Reveal a Role of Plasmodium falciparum Osmiophilic Bodies in Malaria Parasite Transmission. Mol Cell Proteomics. 2016 15(10):3243-3255.
Other associated proteins
PFID | Formal Annotation |
---|---|
PF3D7_1136900 | subtilisin-like protease 2 |
PF3D7_1214800 | conserved Plasmodium protein, unknown function |
PF3D7_1247800 | dipeptidyl aminopeptidase 2 |
PF3D7_1250100 | osmiophilic body protein g377 |