PF3D7_0201900 erythrocyte membrane protein 3

GEXP07 knockout has no effect on the location of other RBC-locating proteins. Immunofluorescence assays were performed using antibodies to PfEMP3, RESA, and MESA. (A and B) anti-PfEMP3 (green) and anti-RESA (green) counter stained with anti-REX1 (red). (C) Anti-MESA (red) counter stained with anti-KAHRP (green). Nuclei were stained with DAPI (blue). Projections of Z stacks are shown. Scale bar = 5 μm. (D to F) Quantification of the mean fluorescence intensity (MFI) of PfEMP3, RESA, and MESA (unpaired t test; = ns, not significant). (D) PfEMP3. CS2 n = 50 cells and ΔGEXP07 n = 42 cells. (E) RESA. CS2 n = 24 cells and ΔGEXP07 n = 54 cells. (F) MESA. CS2 n = 16 cells and ΔGEXP07 n = 15 cells. Fluorescence values are in arbitrary units (a.u). McHugh E, Carmo OMS, Blanch A, Looker O, Liu B, Tiash S, Andrew D, Batinovic S, Low AJY, Cho HJ, McMillan P, Tilley L, Dixon MWA. Role of Plasmodium falciparum Protein GEXP07 in Maurer's Cleft Morphology, Knob Architecture, and P. falciparum EMP1 Trafficking. mBio. 2020 11(2):e03320-19.

Other associated proteins

PFID Formal Annotation
PF3D7_0102200 ring-infected erythrocyte surface antigen
PF3D7_0202000 knob-associated histidine-rich protein
PF3D7_0500800 erythrocyte membrane protein 2 mature parasite-infected erythrocyte surface antigen
PF3D7_0935900 ring-exported protein 1
PF3D7_1301700 cx3cl1-binding protein 2, gametocyte exported protein 7, GEXP7
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