GEXP07 knockout has no effect on the location of other RBC-locating proteins. Immunofluorescence assays were performed using antibodies to PfEMP3, RESA, and MESA. (A and B) anti-PfEMP3 (green) and anti-RESA (green) counter stained with anti-REX1 (red). (C) Anti-MESA (red) counter stained with anti-KAHRP (green). Nuclei were stained with DAPI (blue). Projections of Z stacks are shown. Scale bar = 5 μm. (D to F) Quantification of the mean fluorescence intensity (MFI) of PfEMP3, RESA, and MESA (unpaired t test; = ns, not significant). (D) PfEMP3. CS2 n = 50 cells and ΔGEXP07 n = 42 cells. (E) RESA. CS2 n = 24 cells and ΔGEXP07 n = 54 cells. (F) MESA. CS2 n = 16 cells and ΔGEXP07 n = 15 cells. Fluorescence values are in arbitrary units (a.u). McHugh E, Carmo OMS, Blanch A, Looker O, Liu B, Tiash S, Andrew D, Batinovic S, Low AJY, Cho HJ, McMillan P, Tilley L, Dixon MWA. Role of Plasmodium falciparum Protein GEXP07 in Maurer's Cleft Morphology, Knob Architecture, and P. falciparum EMP1 Trafficking. mBio. 2020 11(2):e03320-19.
Other associated proteins
PFID | Formal Annotation |
---|---|
PF3D7_0102200 | ring-infected erythrocyte surface antigen |
PF3D7_0202000 | knob-associated histidine-rich protein |
PF3D7_0500800 | erythrocyte membrane protein 2 mature parasite-infected erythrocyte surface antigen |
PF3D7_0935900 | ring-exported protein 1 |
PF3D7_1301700 | cx3cl1-binding protein 2, gametocyte exported protein 7, GEXP7 |