Subcellular localization of E120-GFP and E500-GFP in transgenic 3D7 parasite lines. Immuno-electron microscopy studies of 3D7-E120 the N-terminal 120 amino acids attached to GFP (A) and 3D7-E500 the N-terminal 500 amino acids attached to GFP. (B) P. falciparum transgenic parasites labelled with rabbit αGFP antibody. In both cases gold particles were detected in association with the RBC membrane and electron-dense material within the RBC cytoplasm (closed arrows). In 3D7-E120 transfectants the GFP chimera seems to associate underneath knob structures (open arrow) similar to wild-type PfEMP3. However, whereas E500-GFP can be seen associated with Maurer’s clefts, E120-GFP does not associate with these structures. Bars represent 100 nm.
Knuepfer E, Rug M, Klonis N, Tilley L, Cowman AF. Trafficking determinants for PfEMP3 export and assembly under the Plasmodium falciparum-infected red blood cell membrane. Mol Microbiol. 2005 58:1039-53. Erratum in: Mol Microbiol. 2006 59:722.