Localization of PfAK2. (A) Co-localization of PfAK2-GFP with serine-rich protein (SERA5). (B) A comparison the localization of PfAK2-GFP with Bodipy-TR-ceramide shows that the loops contain membranous material. (C) Co-localization of PfAK2-GFP with Plasmodium falciparum exported protein (Exp1) by using an anti-Exp1 antibody. Scale bar, 3 mm. PfAK2 localized to a ring-like structure around the 264 parasite, and ‘‘loops’’ apparently connected to the PVM. PfAK2-GFP co localizes with the PV resident protein SERA5 which seems to be excluded from the ‘‘loops’’ suggesting that PfAK2-GFP in actual fact associates with the membrane of the PV, rather than being found in a soluble state in the PV lumen.

Ma J, Rahlfs S, Jortzik E, Heiner Schirmer R, Przyborski J, Becker K. Subcellular localization of adenylate kinases in Plasmodium falciparum. FEBS Lett. 2012 Jul 17. [Epub ahead of print]