IFA analysis of DMSO and RAP-treated pficm1:cKO schizonts in parallel to the parental B11 line. Cells were counterstained with DAPI and antibodies to the PV marker SERA5. Scale bar 5 μM. Immunofluorescence (IFA) analysis of dimethyl sulfoxide (DMSO)– and RAP-treated schizonts of line pficm1:cKO alongside a WT control did show evidence of protein regulation but the very low levels of protein expression prevented us from localizing the protein.

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