Plasmodium falciparum expressing apicopast-targeted GFP in human erythrocytes. A) ACPleader-GFP expressing parasites co-stained for mitochondria (red) [note the right erythrocyte contains multiple ( 5) infections]. B) detection of GFP in the apicoplast (arrowhead) by immunoglod labeling of ultrarhin sections of ACPleader¬-GFP expressing parasites. C) Green fluorescence of FabH¬leader-GFP expressing parasites. D) Schematic of parasite with major organelles. The parasite is contained within the parasitophorous vacuole (PV) inside the erythrocyte. Arrowhead, apicoplast; N, nucleaus; FV, food v acuole; M, mitochondrion. White scale bar 5 mm Black scale bar 1 mm.

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