PfDOZI::GFP localizes to the cytosolic granules in asexual and sexual blood-stage parasites. a, b Representative live images of the PfDOZI::GFP parasites in asexual erythrocytic stages a and b stage II–V gametocytes. R, ring; ET, early trophozoite; LT, late trophozoite; S, schizont. Nuclei were counter-stained with DAPI. The numbers indicate the co-localization correlation coefficient between the GFP and DAPI fluorescence signals. Scale bar = 2 μm. c, d Co-localization of PfDOZ with Alba4 (c) and PABP1 (d) detected by IFA with the respective antibodies. T, trophozoite; S, schizont; Mz, merozoite; Gc, gametocyte. Nuclei were counterstained with DAPI. The numbers indicate the co-localization correlation coefficient between the GFP and Alba4 or PABP1 fluorescence signals. DIC, differential interference contrast microscopy. Scale bar = 2 μm. All experiments in this figure were repeated three times independently with similar results. Min H, Liang X, Wang C, Qin J, Boonhok R, Muneer A, Brashear AM, Li X, Minns AM, Adapa SR, Jiang RHY, Ning G, Cao Y, Lindner SE, Miao J, Cui L. The DEAD-box RNA helicase PfDOZI imposes opposing actions on RNA metabolism in Plasmodium falciparum. Nat Commun. 2024 15(1):3747.