PF3D7_0414600 acylated pleckstrin-homology domain-containing protein, putative

PF3D7_1459400 protein expression in gametocytes. Methanol-fixed P. falciparum 3D7 gametocytes were stained with a-PF3D7_1459400 -P2 and -P3 rabbit antibodies (red), (1:50) and a-Pfs48/45 rabbit antibody (green), (1:100). (i–ii): a-PF3D7_1459400 P2 rabbit antibody (red), and a-Pfs48/45 mouse antibody (green) stained different stages of gametocytes but a-PF3D7_1459400 peptide 3 rabbit antibody did not show any discernible staining pattern on gametocytes. (iii). Dual immunofluorescence imaging using a-PfAPH rat antibody (1:50); red and a-P2 rabbit antibody (1:50); green indicated that both PfAPH and PF3D7_1459400 native proteins overlap in the cytosolic compartment in gametocytes. Exposure times were identical for all images of the same channel. Secondary antibodies used are Alexa 568-conjugated goat a-rabbit IgG (red), (1:100), Alexa 568-conjugated goat a-rat IgG (red), (1:100); Life technologies, Eugene, USA). Nuclei were stained with DAPI (blue). BF: Bright field; DAPI: 4,6-diamidino-2-phenylindole.

Amlabu E, Nyarko PB, Opoku G, Ibrahim-Dey D, Ilani P, Mensah-Brown H, Akporh GA, Akuh OA, Ayugane EA, Amoh-Boateng D, Kusi KA, Awandare GA. Localization and function of a |Plasmodium falciparum protein (PF3D7_1459400) during erythrocyte invasion. Exp Biol Med (Maywood). 2020 5:1535370220961764.

Other associated proteins

PFID Formal Annotation
PF3D7_1459400 conserved Plasmodium protein, unknown function
img