PF3D7_0415800 phil1-interacting candidate pic3

Localization of PhIL1 interacting candidates (PIC) using endogenous GFP tagging. (a) PIC1 (PF3D7_1229300), (b) PIC2(PF3D7_0822900), (c) PIC4 (PF3D7_0308300), (d) PIC3 (PF3D7_0415800), (e): PIC6 (PF3D7_0530300): On top a schematic representation of the protein (protein length indicated as number of amino acids) with putative protein domains (blue, transmembrane domain; green, signal peptide; red, Zinc Finger domain (ZFD) or RBD (RNA binding domain). Localization of PIC-GFP fusion proteins in schizonts and free merozoites are shown in the middle panel, the PCR-based confirmations of the correct insertion of GFP-encoding integration plasmid into the targeted loci on the bottom. Ladder size indicated in base pairs (bp). gDNA from parental 3D7 was used as control. (f) Localization of PIC5-mCherry (PF3D7_1310700) fusion proteins episomally expressed under the control of the late-stage promoter ama1 in schizonts and free merozoites. Nuclei stained with Hoechst-33,342 or DAPI. Zoom factor: 400%. Scale bar 2μm. KI, knock-in. Wichers JS, Wunderlich J, Heincke D, Pazicky S, Strauss J, Schmitt M, Kimmel J, Wilcke L, Scharf S, von Thien H, Burda PC, Spielmann T, Löw C, Filarsky M, Bachmann A, Gilberger TW. Identification of novel inner membrane complex and apical annuli proteins of the malaria parasite Plasmodium falciparum. Cell Microbiol. 2021 23(9):e13341. PMID: 33830607.

Other associated proteins

PFID Formal Annotation
PF3D7_0308300 phil1-interacting candidate pic4
PF3D7_0530300 phil1-interacting candidate pic6
PF3D7_0822900 phil1-interacting candidate pic2
PF3D7_1229300 phil1-interacting candidate pic1
PF3D7_1310700 PhIL1-interacting protein PhIP
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