Mutational analysis of the minimal sequence requirements for IMC association of PfISP3. E. Substitution of D10 with a positively charged lysine re-directs the GFP-fusion protein to the periphery of the nascent merozoites. The zoom highlights the additional association with the food vacuole membrane characteristic for plasma membrane proteins (E1). Co-localization with the IMC marker GAPM2. Nascent IMC (anti-GAPM2, red) is clearly distinguishable from the peripheral associated 10PfISP3D10K-GFP in early stages (T1) and congruent in late stages (T3).

Wetzel J, Herrmann S, Swapna LS, Prusty D, Peter AT, Kono M, Saini S, Nellimarla S, Wong TW, Wilcke L, Ramsay O, Cabrera A, Biller L, Heincke D, Mossman K, Spielmann T, Ungermann C, Parkinson J, Gilberger TW. The role of palmitoylation for protein recruitment to the inner membrane complex of the malaria parasite. J Biol Chem. 2014 Nov 25. PMID: