Schematic overview of the experiment and Giemsa staining demonstrate that parasites lacking functional PfVsp45 are arrested at the trophozoite stage upon rapalog treatment at 0 to 6 hpi.. (C and D) IFA of PfVsp45 KS parasites ± rapalog (24 hours). IMC biogenesis is inhibited when Vsp45 is mis-localized. GAPM2, parasite IMC. The schizont plasma membrane fails to engulf merozoites when Vsp45 is mis-localized. MSP1, parasite plasma membrane.

Bisio H, Chaabene RB, Sabitzki R, Maco B, Marq JB, Gilberger TW, Spielmann T, Soldati-Favre D. The ZIP Code of Vesicle Trafficking in Apicomplexa: SEC1/Munc18 and SNARE Proteins. mBio. 2020 Oct 20;11(5):e02092-20.