GFP-PfRab1A fluorescence is found as discrete loci in living cells.

A. GFP fluorescence is observed as discrete loci termed PfRab1A bodies in trophozoite and schizont stage parasites expressing GFP-PfRab1A. B. GFP-PfRab18 fluorescence is diffuse and closely associated with the nuclei. C. C-terminal HA-tagged PfRab1A is distributed equally throughout the parasite cytoplasm. D. Infection by parasites expressing GFP-PfRab1A is less efficient than parasites expressing GFP-PfRab18 from the same promoter. Asterisks show significant differences for each time (p ≤ 0.01) using Student’s unpaired two-tailed t-test. E. While not associated with the nucleus, the number of GFP-PfRab1A loci is proportional to the number of nuclei in the cell. Morse D, Webster W, Kalanon M, Langsley G, McFadden GI. Plasmodium falciparum Rab1A Localizes to Rhoptries in Schizonts. PLoS One. 2016 11(6):e0158174.