Subcellular localization of six putative P. falciparum transporters during asexual blood stage development. (B) PfFVRT1-GFP-glmS, (C) PfZIP1-GFP-glmS, (D) PfCDF-GFP-glmS, (E) PfDMT2-GFP-glmS,
(F) PfMFS6-GFP-glmS and (G) PfPMRT1-GFP-glmS by live-cell microscopy in ring, trophozoite and schizont stage parasites. Nuclei were stained with Hoechst-33342. (H) Co654 localization of the GFP-tagged putative transporters with marker proteins P40PX-mCherry
(food vacuole), ACP-mCherry (apicoplast), Lyn-mCherry (parasite plasma membrane), ARO656 mCherry (rhoptry) and AMA1-mCherry (microneme) as indicated. Nuclei were stained with 657 Hoechst- 33342. Scale bar, 2 μm. Wichers JS, Mesén-Ramírez P, Fuchs G, Yu-Strzelczyk J, Stäcker J, von Thien H, Alder A, Henshall I, Liffner B, Nagel G, Löw C, Wilson D, Spielmann T, Gao S, Gilberger TW, Bachmann A, Strauss J. PMRT1, a Plasmodium-Specific Parasite Plasma Membrane Transporter, Is Essential for Asexual and Sexual Blood Stage Development. mBio. 2022 13(2):e0062322. PMID: 35404116
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Other associated proteins
PFID | Formal Annotation |
---|---|
PF3D7_0208500 | acyl carrier protein |
PF3D7_0414900 | armadillo-domain containing rhoptry protein |
PF3D7_0715900 | zinc transporter, putative |
PF3D7_1135300 | conserved Plasmodium membrane protein, unknown function |
PF3D7_1440800 | major facilitator superfamily domain-containing protein, MFS6 |