PF3D7_0523800 divalent metal transporter, putative

Subcellular localization of six putative P. falciparum transporters during asexual blood stage development. (A) Schematic representation of endogenous tagging strategy using the selection-linked integration system (SLI). Pink, human dihydrofolate dehydrogenase (hDHFR); gray, homology region (HR); green, green fluorescent protein (GFP) tag; dark gray, T2A skip peptide; blue, neomycin resistance cassette; orange, glmS cassette. Stars indicate stop codons, and arrows depict primers (P1 to P4) used for the integration check PCR. (B to G) Localization of (B) PfFVRT1-GFP-glmS, (C) PfZIP1-GFP-glmS, (D) PfCDF-GFP-glmS, (E) PfDMT2-GFP-glmS, (F) PfMFS6-GFP-glmS, and (G) PfPMRT1-GFP-glmS by live cell microscopy in ring, trophozoite, and schizont stage parasites. Nuclei were stained with Hoechst 33342. (H) Colocalization of the GFP-tagged putative transporters with marker proteins P40PX-mCherry (food vacuole), ACP-mCherry (apicoplast), Lyn-mCherry (parasite plasma membrane), ARO-mCherry (rhoptry), and AMA1-mCherry (microneme) as indicated. Nuclei were stained with Hoechst 33342. Scale bar, 2 μm. Wichers JS, Mesén-Ramírez P, Fuchs G, Yu-Strzelczyk J, Stäcker J, von Thien H, Alder A, Henshall I, Liffner B, Nagel G, Löw C, Wilson D, Spielmann T, Gao S, Gilberger TW, Bachmann A, Strauss J. PMRT1, a Plasmodium-Specific Parasite Plasma Membrane Transporter, Is Essential for Asexual and Sexual Blood Stage Development. mBio. 2022 13(2):e0062322. PMID: 35404116

Other associated proteins

PFID Formal Annotation
PF3D7_0609100 zinc transporter zip1, putative
PF3D7_0716900 drug/metabolite transporter dmt2
PF3D7_1135300 conserved Plasmodium membrane protein, unknown function
PF3D7_1440800 major facilitator superfamily domain-containing protein, MFS6
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