Expression and sub-cellular localization patterns of H3K9Ac and H3K14Ac histone marks. (D) Perinuclear localization pattern of acetylated H3K9Ac marks. Immunofluorescence analysis using antibodies specific for H3K9Ac confirms horseshoe-shaped pattern around nuclear periphery. H3K9Ac also localizes as sharp 3-7 perinuclear foci in majority of the parasites. (E) Schematic representation of H3K9Ac mark in parasite nuclei. (F) Localization pattern of H3K14Ac mark in the parasite nuclei. Unlike H3K9Ac, H3K14Ac signal shows even distribution on the DAPI. (G) Schematic diagram of H3K14Ac marks in the parasite nuclei. Scale bar measures 1 μM. DAPI stains ring nuclei.

Srivastava S, Bhowmick K, Chatterjee S, Basha J, Kundu TK, Dhar SK. Histone H3K9 acetylation level modulates gene expression and may affect parasite growth in human malaria parasite Plasmodium falciparum. FEBS J. 2014 Sep 22. [Epub ahead of print]