(B) CAF1 and CAF2 showed co-localization during ring and trophozoite stages of parasite. (C) Co-localization of PfCAF1 and H3K56Ac in infected red blood cells. Results show co-localization of PfCAF1 and PfCAF2 in the parasites (Fig. 2B). These results suggest that PfCAF1 and PfCAF2 interact and co-localize from early to late stages of parasite development, which coincide with the DNA replication stage of the parasite. During nucleosome assembly, newly formed histones are deposited by CAF1 behind the replication fork onto the chromatin. Previous studies suggested that newly synthesized histones have to be methylated and acetylated before delivering to the chromatids. conservation of H3K56Ac mark for newly synthesized histones in P. falciparum.

Gupta MK, Agarawal M, Banu K, Reddy KS, Gaur D, Dhar SK. Role of Chromatin assembly factor 1 in DNA replication of P. falciparum. Biochem Biophys Res Commun. 2017 Nov 26. pii: S0006-291X(17)32310-0.