Inducible knockdown of REX1 using the glmS ribozyme system decreases the number of Maurer's cleft puncta. B,D. Immunofluorescence
microscopy of paraformaldehyde-fixed infected RBCs probed with Maurer’s cleft marker anti-
MAHRP1 (green) and anti-HA or anti-KAHRP (red). Nuclei are stained with DAPI (blue). Scale bar = 3 μm. C. Quantitation of numbers of Maurer’s clefts produced by REX1_KD transfectants in the presence and absence of 2.5 mM GlcN. The mean number of Maurer’s clefts produced per singly nucleated infected RBC was determined by counting SBP1-labelled puncta in at least 10 cells. Error bars = S.D. These puncta correspond to individual Maurer’s cleft cisternae that have a distributed organization within the RBC cytoplasm
McHugh E, Batinovic S, Hanssen E, McMillan PJ, Kenny S, Griffin MD, Crawford S, Trenholme KR, Gardiner DL, Dixon MW, Tilley L. A repeat sequence domain of the ring-exported protein-1 of Plasmodium falciparum controls export machinery
architecture and virulence protein trafficking. Mol Microbiol. 2015 Aug 24. [Epub ahead of print]
Other associated proteins
PFID | Formal Annotation |
---|---|
PF3D7_0202000 | knob-associated histidine-rich protein |
PF3D7_1370300 | membrane associated histidine-rich protein |