Fig 1 (left) PfARK1 is expressed in a subset of nuclei during schizogony. (A) Localization of PfARK1–GFP throughout intra-erythrocytic asexual development as assessed by live cell fluorescence microscopy. Numbers in brackets indicate the age range (hours post invasion) of the synchronous parasite samples imaged at consecutive time points across intra-erythrocytic development. R, ring stage; T, trophozoite; S, schizont; seg., segmented. (B, C) Localization of PfARK1-GFP, centrin, and α/β-tubulin (B) or CENH3 (C) in schizonts as assessed by IFAs. Representative images are shown in each panel. DNA was stained with Hoechst (live) or 4′,6-diamidino-2-phenylindole (DAPI) (IFA). DIC, differential interference contrast. A1, PfARK1; CEN, centrin; TUB, α/β-tubulin; CH3, CENH3; i/ii, zoom selections. Fig 2 (right) PfARK2 is expressed in all nuclei during schizogony. (A) Localization of PfARK2–GFP throughout intra-erythrocytic asexual development as assessed by live cell fluorescence microscopy. Numbers in brackets indicate the age range (hours post invasion) of the synchronous parasite samples imaged at consecutive time points across intra-erythrocytic development. R, ring stage; T, trophozoite; S, schizont; seg., segmented. (B, C) Localization of PfARK2–GFP, centrin, and α/β-tubulin (B) or CENH3 (C) in schizonts as assessed by IFAs. Representative images are shown in each panel. DNA was stained with Hoechst (live) or DAPI (IFA). DIC, differential interference contrast. A2, PfARK2; CEN, centrin; TUB, α/β-tubulin; CH3, CENH3; i, zoom selections i
Other associated proteins
PFID | Formal Annotation |
---|---|
PF3D7_0107000 | centrin-1 |
PF3D7_0309200 | serine/threonine protein kinase ark2, putative |
PF3D7_0903700 | alpha tubulin 1 |
PF3D7_1008700 | tubulin beta chain |
PF3D7_1333700 | histone H3-like centromeric protein CSE4 |