TEM analysis of immunogold labelling of PfEMP1 in SLO-permeabilized parasitized RBCs. A–F. A4 clone parasitized RBCs were lysed with SLO and incubated with anti-ATS serum followed by 5 nm gold-labelled anti-rabbit IgG, then fixed in 4% glutaraldehyde. Gold particles decorating Maurer’s cleft extensions are marked with arrowheads. Gold particles decorating knobs on the RBC membrane are marked with arrows. Maurer’s clefts in partial tangential section are marked with asterisks. Bars represent 100 nm.
Kriek N, Tilley L, Horrocks P, Pinches R, Elford BC, Ferguson DJ, Lingelbach K, Newbold CI. Characterization of the pathway for transport of the cytoadherence-mediating protein, PfEMP1, to the host cell surface in malaria parasite-infected erythrocytes. Mol Microbiol. 2003 50:1215-27. Copyright John Wiley & Sons Ltd. 2010