Immuno-EM. Paraformaldehyde-fixed, EqtII-permeabilized PfEMP1–GFP transfectants were labelled with anti-PfEMP1 (E) serum or anti-GFP serum (F, G), followed by Protein A–gold (6 nm conjugate). Abbreviations: MC, Maurer’s clefts; PM, RBC plasma membrane; PVM, PV membrane. Scale bars: (A, B) 1 μm; (C) 100 nm; (D) 2.5 μm; (E) 50 nm; and (F) 100 nm.
Jackson KE, Spielmann T, Hanssen E, Adisa A, Separovic F, Dixon MW, Trenholme KR, Hawthorne PL, Gardiner DL, Gilberger T, Tilley L. Selective permeabilization of the host cell membrane of Plasmodium falciparum-infected red blood cells with streptolysin O and equinatoxin II. Biochem J. 2007 403:167-75. © the Biochemical Society