Disruption of the RON3 C-terminal fragment function leads to unusual protein export by PTEX translocon. Ring-infected RBCs from RON3-DDD parasites were cultivated with or without TMP (con and ES in Figure 2A). IFA images of ring-infected cells with (A) anti-RESA or (B) anti-SBP1 with anti-EXP2 antibodies. An image is shown for an export or no export pattern of A) RESA or B) SBP1. RESA and SBP1 staining are shown in green, and EXP2 staining is shown in red. The nuclei staining (DAPI) is shown in blue. DIC, differential interference contrast. Bars, 5 μm.