Indirect immunofluorescence assay with affinity purified rabbit anti-MSP5 antibodies showing surface labelling of P. falciparum schizonts (left panel) and individual merozoites (right panel) with FITC-conjugated sheep anti-rabbit Ig secondary antibody. No fluorescence was detected in ring-stage, but schizonts and free merozoites were observed to fluoresce. Staining was observed in a rim-like pattern around the circumference of the merozoite both within schizonts and as free merozoites.

Wu T, Black CG, Wang L, Hibbs AR, Coppel RL. Lack of sequence diversity in the gene encoding merozoite surface protein 5 of Plasmodium falciparum. Mol Biochem Parasitol. 1999 103:243-50. Copyright Elsevier 2009.