Indirect immunofluorescence studies with rabbit anti-MSP-4 antibodies. P. falciparum-infected erythrocytes were stained with antisera from rabbits immunized with an N-terminal fragment of MSP-4 (from strain w2mef) expressed as a fusion with GST (VM912C). Staining of schizonts shows a typical bunch-of-grapes appearance, which is characteristic of merozoite surface proteins.

Marshall VM, Silva A, Foley M, Cranmer S, Wang L, McColl DJ, Kemp DJ, Coppel RL. A second merozoite surface protein (MSP-4) of Plasmodium falciparum that contains an epidermal growth factor-like domain. Infect Immun. 1997 65:4460-7.