Localization of PfDDX17 A. Preimmune staining (i) Phase contrast (TD); (ii) DAPI; (iii) All merged. B. Ring stage, C. Merozoite stage, D. Trophozoite stage, E. Schizont stage, F. Mature Schizont, (i) TD, (ii) DAPI, (iii) PfDDX17, (iv) merged (DAPI +PfDDX17). G. The transcript level of PfDDX17 of different intraerythrocytic stages of P. falciparum 3D7 strain using Real time PCR. Error bars represent standard deviation. DAPI and fluorescent conjugated anti-rabbit antibody were used to detect nucleus and PfDDX17, respectively. In all the stages DDX17 is localised mainly in the cytoplasm. The expression of PfDDX17 is less in ring stage (B). During the trophozoite stage the expression level significantly increased and appeared on the iRBC membrane and cytoplasm (D). Similar to the trophozoite stage PfDDX17 localised in the cytoplasm in the schizont and bursting schizont stage (E–F). The pre-immune serum did not detect DDX17 in the parasite.

Sourabh S, Chauhan M, Yasmin R, Shehzad S, Gupta D, Tuteja R. Plasmodium falciparum DDX17 is an RNA helicase crucial for parasite development. Biochem Biophys Rep. 2021 26:101000.