Protein expression and localization of PfCRISP in sexual stages. IFAs were performed on gametocytes in solution in microcentrifuge tubes using anti-PfCRISP antisera (in green) in combination with PfCDPK4 (in red). IFAs were performed on gametocytes 10 min post activation and free microgametes on thin smears using anti-PfCRISP antisera (in green) in combination with PfCDPK4 (in red). The parasite nucleus was stained with DAPI (blue). White arrow marks one end of the microgamete. Scale bar = 5 μm. (D) IFAs were performed on gametocytes on thin smears using anti-PfCRISP antisera (in green) in combination with Pfg377 (in red). The parasite nucleus was stained with DAPI (blue). Scale bar = 5 μm.

Kumar S, Leeb AS, Vaughan AM, Kappe SHI. Plasmodium falciparum Cysteine Rich Secretory Protein uniquely localizes to one end of male gametes. Mol Biochem Parasitol. 2022 248:111447.