Brefeldin A treatment induces accumulation of HSP101 in the ER. (A) Representative IFA images of HSP101-HAglmS parasites treated with 0, 2, and 10 μM Brefeldin A (BFA) for 24 hours starting from the ring stage. Cells were fixed and stained with anti-HA (HSP101) and anti-ERC antibodies (ER marker). (C) Older ring stage parasites (16–20 hours post.

Gabriela M, Matthews KM, Boshoven C, Kouskousis B, Jonsdottir TK, Bullen HE, Modak J, Steer DL, Sleebs BE, Crabb BS, Koning-Ward TF, Gilson PR. A revised mechanism for how Plasmodium falciparum recruits and exports proteins into its erythrocytic host cell. PLoS Pathog. 2022 18(2):e1009977.