Intra-parasite HSP101 co-localises with P5 Lys Hyp1-Nluc-mDHFR-3xFLAG reporter construct in the endoplasmic reticulum of the parasite. (A) Representative IFA images (n = 3 independent replicates) of parasites expressing Hyp1-Nluc-mDHFR-3xFLAG constructs with WT P5 glutamate reporter exported into the erythrocyte and the mutant P5 Lys Hyp1-Nluc-mDHFR-3xFLAG reporter trapped in the parasite ER. To visualise translocon components, cells were probed with anti-EXP2 and anti-HA (to visualise HA-tagged HSP101). Anti-PfERC was used Nluc to visualise the parasite’s ER. Hyp1-Nluc-mDHFR-3xFLAG reporter proteins were localised using either anti-Nluc or anti-FLAG antibodies. The red bar in the schematic picture of the construct indicates the PEXEL motif and variations thereof. The blue bar indicates the transmembrane signal peptide. Scale bars, 5 μm. DIC, Differential Interference Contrast. DAPI was used to stain parasite nuclei

Gabriela M, Matthews KM, Boshoven C, Kouskousis B, Jonsdottir TK, Bullen HE, Modak J, Steer DL, Sleebs BE, Crabb BS, Koning-Ward TF, Gilson PR. A revised mechanism for how Plasmodium falciparum recruits and exports proteins into its erythrocytic host cell. PLoS Pathog. 2022 18(2):e1009977.