Pf3D7-1437000 depletion does not affect protein export. (A) Parasites were synchronized at the early ring stage, maintained in medium as shown with 10 μM trimethoprim (+TMP) or an equal volume of DMSO (−TMP), or 100 nM aTc (+aTc) or an equal volume of DMSO (−aTc). Parasites were fixed 24 h after invasion as trophozoites and stained to compare protein export in Hsp101-DD +/−TMP and 1437APT +/−aTc. Exported proteins are marked by antiHRP2 or anti-FIKK4.2, each shown in green; the parasite cytosol is marked by anti-aldolase, shown in red; parasite nucleus marked by DAPI is in blue. Experiment was performed three times; representative images are shown. Scale bars: 5 μm.

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