PfERC knockdown inhibits SUB1 and MSP1 processing. (E), anti-AMA1 (F), anti-RAP1 (G) antibodies (green), parasite nucleus stained with DAPI (blue), and fluorescence merge images of the schizonts incubated with GlcN for 48hrs (n=2 biological replicates). Scale bar 2µm. Small arrowheads point to rhoptries, large arrowheads to micronemes, and white arrowheads to PVM fragments. Scale bar, 200nm. PfERC knockdown inhibits SUB1 and MSP1 processing. (H) Representative TEM images of synchronized PfERC-glmS and PfERC-M9 schizonts grown for 48 h with GlcN and incubated with C1 for 4 h (n = 2 biological replicates). Small arrowheads (white) point to micronemes, while large arrowheads (black) point to rhoptries. Bar, 200 nm.

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