Subcellular localization of six putative P. falciparum transporters during

asexual blood stage development. (B–G) Localization of (B) PfFVRT1-GFP-glmS, (C) PfZIP1-GFP-glmS, (D) PfCDF-GFP-glmS, (E) PfDMT2-GFP-glmS, (F) PfMFS6-GFP-glmS and (G) PfPMRT1-GFP-glmS by live-cell microscopy in ring, trophozoite and schizont stage parasites. Nuclei were stained with Hoechst-33342. (H) Co654 localization of the GFP-tagged putative transporters with marker proteins P40PX-mCherry (food vacuole), ACP-mCherry (apicoplast), Lyn-mCherry (parasite plasma membrane), ARO656 mCherry (rhoptry) and AMA1-mCherry (microneme) as indicated. Nuclei were stained with Hoechst- 33342. Scale bar, 2 μm.