Immunofluorescence assays of parasites expressing wild-type PV6 or wild-type PV6 plus PV6 K64A. The PVM was visualized with an anti-EXP1 antibody. PV6 was detected in the erythrocyte cytosol only in erythrocytes infected with parasites expressing the PV6 K64A mutant. See Fig. S3 for additional images. The scale bar represents 5 µm.

Fréville A, Ressurreição M, van Ooij C. Identification of a non-exported Plasmepsin V substrate that functions in the parasitophorous vacuole of malaria parasites. mBio. 2023 11:e0122323.