Confocal immunofluorescence co-localization of PfERC with the ER-located protein, Pfgrp (PFI0875w). Mature trophozoite-infected erythrocytes (D10 strain, Panels A–D or FCR3strain, Panels E–F) were double-labeled with mouse antiserum against recombinant Pfs40:PfERC produced in either yeast (Panels A,E) or E. coli (Panel C), followed by FITC-conjugated anti-mouse antibodies and a rabbit anti-Pfgrp antiserum followed by a Cy3-conjugated anti-rabbit antibodies (Panels B,D,F). An optical slice was collected through the centre of the parasites by confocal microscopy. Dual images were collected simultaneously and are displayed as isolated fluorescein fluorescence (Panels A,C,E) and isolated Cy3 fluorescence (Panels B,D,F) of the same cell. Labeling pattern suggests localization of PfERC within structures within the parasite cytoplasm. Staining profile for the known ER-resident protein, Pfgrp exactly co-localize with PfERC.

La Greca N, Hibbs AR, Riffkin C, Foley M, Tilley L. Identification of an endoplasmic reticulum-resident calcium-binding protein with multiple EF-hand motifs in asexual stages of Plasmodium falciparum. Mol Biochem Parasitol. 1997 89:283-93. Copyright Elsevier